What is ICSI?
- What’s the process between thawing the sperm and putting them together with the eggs? Do you check the sperms after thawing? And if yes, what would you be looking at? Separately, assuming you were prepared to do a conventional insemination but the sperms are not as active as they should be or the count is low, would you be able to switch to ICSI?
Frozen sperm is thawed and observed under a microscope. We take note of the concentration and motility to calculate the total number of motile sperm in the sample. When performing conventional insemination the oocyte and sperm are placed in the same dish and allowed to co-incubate overnight. If the parameters fall below normal values we notify your doctor and proceed with ICSI. Please note that we recommend ICSI when using frozen sperm.
- When do you start the sperm thawing, immediately after the egg retrieval or before? I am asking because there is a possibility that no egg is retrieved.
The sperm is thawed after the retrieval and when the maturity status of the oocyte is known.
- If doing ICSI, what’s the process for picking the sperm? You would get the one you could catch and assuming it is a normal looking one, it will be the one? Do you use any techniques to filter some of the lower quality ones before the election?
The sperm is observed under a high power microscope and is selected by an embryologist based on morphology and forward progression.